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The second reaction cycle in NO synthesis involves O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to generate ferric enzyme, NO, citrulline, and water as items (Fig. 9) - spectrophotometers. As drawn in Fig - https://pblc.me/pub/3fc0b3e264b77b. 9, the reaction might likewise include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this might be since the H4B extreme serve as an electron sink in a later point in the response, so as to allow NOS to, Fig. 9. A model for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that reacts with NOHA.